Chrysanthemum plants regenerated from ovaries: a study on genetic and phenotypic variation

• DOI: 10.3906/bot-1707-19
• Publication Date: 2018-05-03T12:44:08Z
• Authors: Natalia Miler; Iwona Jędrzejczyk
• Keywords: Somaclonal variation; Ornamental plant

Chrysanthemum (Chrysanthemum × grandiflorum /Ramat./Kitam.) is the second most popular ornamental plant in global flower industry, and there still a demand for novelty, which forces breeders to search new sources of variation. The aim present study was evaluation phenotypic as well genetic variation chrysanthemum plants regenerated from ovaries vitro. In first vegetative season evaluated glasshouse, nine variants (16.36%) 46 (83.6%) true-totype were observed. included variegated, marble-like, lighter-green leaves, changes morphology inflorescences ligulate florets, shape corymb. Variants with variegated marble-like leaves unstable. All 55 regenerants had same ploidy level (2n = 6x) control plants, estimated by flow cytometry. Genetic analysis based on RAPD-PCR revealed distances ranging 0.93% 7.69% between plants. It concluded that variable did not originate gynogenic pathway, but they somatic tissue underwent somaclonal