Sesame phyllody disease: its symptomatology, etiology, and transmission in Pakistan

• DOI: 10.3906/tar-0901-23
• Publication Date: 2022-06-27T21:35:49Z
• Authors: KHALID PERVAIZ AK...; GHULAM SARWAR; MATTHEW DICKINSON; MUSHTAQ AHMAD; MUHAMMAD AHSANUL HAQ; SOHAIL HAMEED; MUHAMMAD JAVEED I...
• Keywords: Phyllody; Phytoplasma; Virescence; Sieve tube element

Phyllody is a serious disease of sesame in Pakistan. In the present study investigations were carried out on symptomatology, etiology, and transmission this disease. Floral virescence, phyllody, proliferation are most common symptoms. Sometimes these symptoms accompanied by yellowing, cracking seed capsules, germination seeds formation dark exudates foliage. Shoot apex fasciation has also been occasionally observed, but no phytoplasma DNA detected fasciated plants using PCR assays. Light microscopy hand-cut sections treated with Dienes' stain showed blue areas phloem region phyllody-infected plants. Pleomorphic bodies (phytoplasma structures) observed sieve elements diseased electron (TEM). Amplification characteristic 1250-bp 16S rDNA fragment confirmed that was infected phytoplasma, RFLP profiling sequencing associated had greatest homology to 16SrII-D group phytoplasmas. successfully transmitted grafting dodder (Cuscuta compestris) leafhopper (Orosius albicinctus). Treatment tetracycline-HCl provided temporary recovery from