Using Quantitative Real Time PCR Melt Curve Analysis of Partial CO1 Sequence for Rapid Biotype Differentiation ofBactericera cockerelli(Hemiptera: Triozidae)

• DOI: 10.3958/059.037.0405
• Publication Date: 2012-12-10T11:07:04Z
• Authors: Rebekah I. Chapman; Juan F. Macias-Ve...; Alex P. Arp; Blake R. Bextine
• Keywords: Solanum tuberosum

The potato psyllid, Bactericera cockerelli (Sulc), is a native of North America and occurs from Central to Canada. A newly emerging plant pathogen, Candidatus Liberibacter solanacearum, associated with psyllids in New Zealand, has become major concern many solanaceous crops worldwide. In potato, Solanum tuberosum L., the resulting disease called zebra chip, but similar foliar vascular symptoms have been reported tomato, lycopersicum peppers, Capsicum annuum L. To date, it known that transmit they are primary mode pathogen spread pepper agroecosystems Zealand. Potato rather wide range hosts, including wild plants, which may crop plants. Within insect species, B. cockerelli, two biotypes exist. This differentiation was originally hardiness cold temperatures can be monitored through genotypic variations cytochrome oxidase 1 (CO1) gene. this study, psyllid four locations were delineated by melt temperature analysis following Sybr Green quantitative real-time PCR. Dakota, northern extreme for central psyllids, 100% samples “Central Biotype”; whereas, both (Central Western) found Colorado where seem overlap.