M2 Macrophage/Microglial Cells Induce Activation of Stat3 in Primary Central Nervous System Lymphoma

• DOI: 10.3960/jslrt.51.93
• Publication Date: 2011-11-22T06:11:56Z
• Authors: Yoshihiro Komohara; Hasita Horlad; Koji Ohnishi; Kazutaka Ohta; Keishi Makino; Hiroaki Hondo; Ryuya Yamanaka; Koji Kajiwara; Takafumi Saito; Jun Kuratsu; Motohiro Takeya
• Keywords: CD163; CD68; M2 Macrophage; Tumor-associated macrophage

Primary central nervous system lymphoma (PCNSL) is one of the most aggressive malignant lymphomas with a median survival less than 20~40 months. Interest in signal transducer and activator transcription 3 (Stat3) has increased during past decade because Stat3 activation was found to contribute tumor progression by inducing angiogenesis, immunosuppression, metastasis. We previously demonstrated significant correlation between cells infiltrating anti-inflammatory (M2) macrophages. Here, we focused on phenotypes macrophages/microglial PCNSL cells. The or density M2 macrophage infiltration patient prognosis also evaluated. performed immunostaining for CD68, CD163, CD204, pStat3 using paraffin-embedded specimens obtained from 43 patients. CD163 CD204 served as markers phenotype. Dense CD68+ macrophages all samples. High numbers CD163+ CD204+ were observed 29 25 cases, respectively. enhanced patients who showed denser tissues. In vitro co-culture experiment investigate cell-cell interactions that strongly activated Numbers CD68+, CD163+, tumor-associated (TAMs) not correlated prognosis. However, involvement development several tumors, our present finding macrophage/microglial induced may provide novel insights into pathogenesis. [J Clin Exp Hematopathol 51(2) : 93-99, 2011]