Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

• DOI: 10.4014/jmb.1909.09041
• Publication Date: 2020-04-29T02:49:32Z
• Authors: Yongshi Liu; Qiong Liu; Yanlong Jiang; Wentao Yang; Haibin Huang; Chunwei Shi; Guilian Yang; Chunfeng Wang
• Keywords: Intestinal epithelium; Coronavirus; Enteric bacteria; Enteric virus

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) transmissible gastroenteritis (TGEV), cause high morbidity mortality piglets resulting economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it particularly important to develop a new drug prevent infected with mixed piglets. In this study, we first constructed anchored expression vector CWA (C-terminal cell wall anchor) on L. plantarum. Second, recombinant strains that IFN-λ3 via pgsA (N-terminal transmembrane CWA. Third, demonstrated both possess effects against coronavirus infection intestinal epithelial line J2 (IPEC-J2). However, anchor exhibited more powerful effect than pgsA. Consistent finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced levels IFN-stimulated genes (ISGs) (ISG15, OASL, Mx1) IPEC-J2 did Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies inhibits PEDV TGEV cells, may offer great for use as novel oral agent therapeutic applications combating gastroenteritis. This show suppresses cells.