Produção e caracterização de um marcador potencial de linfócitos T em surubim híbrido (Pseudoplatystoma corruscans X P. fasciatum)

• DOI: 10.4025/actascibiolsci.v26i2.1636
• Publication Date: 2009-07-20T19:07:23Z
• Authors: Roger Beelen; Gilberto Cezar Pa...; Bobbie Boyd; Julio Cesar Garav...; Patricia Mendes G...; Avery Jerald Ains...
• Keywords: Immunogold labelling; Thymocyte

A monoclonal antibody against hybrid surubim thymocytes and circulating T-cells (PST 33) was produced characterized. The mAb obtained by immunizing mice with isolated membrane molecules of thymocytes, whole thymocytes. Flow cytometric analysis showed that PST 33 reacted 87.5% the 34.1% circulating, 19.1% pronephros, 36.6% spleen lymphoid cells. Immunohistochemistry demonstrated 33-immunoreactive cells are mostly present in medullar region thymus, vicinity arterioles spread throughout parenchyma spleen. appeared as a suitable marker for immunohistochemistry, should prove useful studying ontogeny immune system. Immunogold labeling revealed 33+ were lymphoid-like Morphologically they small lymphocytes high nucleus/cytoplasm ratio. Western blotting non-reduced lysates 215 kDa molecule