Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis

• DOI: 10.4047/jap.2015.7.1.21
• Publication Date: 2015-02-17T10:24:51Z
• Authors: Meral Arslan Malkoç; Necla Demİr; Abdulkadir Şengün; Şerife Buket Bozkurt; Sema Sezgin Hakki
• Keywords: Real-time cell analysis; 03 medical and health sciences; 0302 clinical medicine; Cytotoxicity; Dental pulp-derived cells; Original Article; Temporary cements

To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 µL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments.All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results.The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.