Two populations of Ia-like molecules on a human B cell line.

• DOI: 10.4049/jimmunol.125.1.293
• Publication Date: 2022-12-31T00:05:18Z
• Authors: L A Lampson; R Levy
• Keywords: B-Lymphocytes; 0303 health sciences; Radioimmunoassay; Hybrid Cells; Binding, Competitive; Antibodies; Cell Line; Clone Cells; 3. Good health; 03 medical and health sciences; Histocompatibility Antigens; Chemical Precipitation; Humans; Binding Sites, Antibody

Abstract Evidence is presented for two populations of Ia-like molecules on the human B cell line Raji. Two monoclonal antibodies, L203 and L227, are described. Both recognize nonpolymorphic determinants on B cell antigens. Both immunoprecipitate 34,000- and 28,000-dalton material from 125I-labeled extracts of Raji, but L227 precipitates 25,000-dalton material as well. Immunodepletion experiments confirm that the antibodies react with two different populations of molecules.