Amiloride inhibition of DNA synthesis and immunoglobulin production by activated human peripheral blood mononuclear cells is independent of sodium/hydrogen antiport.

• DOI: 10.4049/jimmunol.137.4.1300
• Publication Date: 2022-12-31T03:00:05Z
• Authors: D T Yamaguchi; R Sakai; L Bahn; E J Cragoe; S C Jordan
• Keywords: Amiloride; Sodium–hydrogen antiporter

Activation of sodium/proton (Na+/H+) antiport activity has been shown to occur as an early event in mitogenesis. Because amiloride inhibits Na+/H+ activity, it is hypothesized that mitogenesis may be inhibited by amiloride. In this work, we examined the effect on DNA synthesis measured [3H]thymidine uptake and immunoglobulin (Ig) production ELISA system human peripheral blood mononuclear cells (PBM). Amiloride at 100 microM concentration irradiated Raji cell (*R)-activated phytohemagglutinin-P (PHA-P)-stimulated 50 +/- 11% 72 12%, respectively. IgG was 71% *R-activated PBM. This 92%. known inhibit other pre-replicative cellular functions such protein synthesis, used analogue, dimethylamiloride, which 90% a 1 without inhibition PBM Ig or synthesis. Furthermore, neither PHA-P nor *R-stimulated demonstrated intracellular alkalinization even after 6 hr stimulation. Similarly, T cell-enriched B populations did not show *R activation. Thus, appears activation The due abrogation premitotic events