Studies on the molecular nature of human interleukin 1.

• DOI: 10.4049/jimmunol.138.5.1447
• Publication Date: 2022-12-31T22:02:53Z
• Authors: P E Auron; S J C Warner; A C Webb; J G Cannon; H A Bernheim; K J P W McAdam; L J Rosenwasser; G LoPreste; S F Mucci; C A Dinarello
• Keywords: Chromatofocusing

Adherent human blood monocytes were stimulated with heat-killed Staphylococcus albus or Escherichia coli lipopolysaccharide in the presence of 35S-methionine-, [3H]leucine-, 14C-labeled amino acids. After incubation, interleukin 1 (IL 1) activity supernatant medium was purified over an anti-human IL immunoadsorbent followed by gel filtration and chromatofocusing. The purity assessed fluorography one- two-dimensional SDS-polyacrylamide electrophoresis. Isoelectric chromatofocusing low m.w. proteins (less than 20,000 m.w.) revealed three charged 18,000 species approximate pI's 7, 6, 5, most abundant form at pI 7. During purification procedures, lymphocyte co-mitogenic activity, fever rabbits, prostaglandin E2 release from dermal fibroblasts co-eluted same fractions. In addition, these fractions active when injected into endotoxin-resistant C3H/HeJ mice for production fever, induction serum amyloid A protein, a decrease iron concentration, increase number circulating neutrophils. Fluorography homogeneous bands about which correlated biological activities. specific 6 5 1, as judged ratio T cell to incorporated radiolabeled acid, least 10-fold greater that observed 7 form. This result suggests acid compositions two acidic forms are unrelated species. These results also demonstrate monocyte is predominant synthesized and, furthermore, exhibits multiple properties on various tissues modulating immunologic, inflammatory, metabolic, neurologic functions. Data presented existence high (32,000) pro-IL molecule monocytic intracellular degraded artifactually during isolation lower extracellular serine protease activity. data consistent model secretion first within processed after transport.