Complete Freund's adjuvant-induced T cells prevent the development and adoptive transfer of diabetes in nonobese diabetic mice.

• DOI: 10.4049/jimmunol.150.5.2072
• Publication Date: 2022-12-31T09:27:20Z
• Authors: H Y Qin; M W Sadelain; C Hitchon; J Lauzon; B Singh
• Keywords: NOD mice; Adoptive Cell Transfer; Insulitis

Abstract Insulin-dependent diabetes mellitus is an autoimmune disease that characterized by the destruction of insulin-producing beta cells in islet Langerhans. We have recently reported induction nonobese diabetic (NOD) mice can be prevented a single injection CFA. In this study, we explored cellular basis and time course protection. Since CFA contains mycobacterial cell wall has adjuvant property, investigated protective role mycobacteria young NOD mice. Mice injected with Mycobacterium tuberculosis or bovis (BCG vaccine) at 4 wk age were also found to protected from diabetes. complete protection only achieved administration between 10 age. Draining lymph node spleen CFA-treated transfer Adoptive acutely delayed into irradiated recipient old resistant passive Depletion Thy 1.2+ CD4(+)-bearing T abrogated However, induced cyclophosphamide treatment. thymocytes responded weakly mitogen Con A. treatment, however, restored ability these respond Finally, our results suggest after treatment prevent both effector phases disease.