T cell-dependent loss of proliferative responsiveness to colony-stimulating factor-1 by a murine epidermal-derived dendritic cell line, XS52

• DOI: 10.4049/jimmunol.155.11.5190
• Publication Date: 2022-12-31T12:18:54Z
• Authors: T Kitajima; K Ariizumi; P R Bergstresser; A Takashima
• Keywords: clone (Java method); Epidermis (zoology)

We have reported previously that XS52 cells, a long-term dendritic cell (DC) line established from mouse epidermis, proliferate maximally in response to CSF-1, and cells expanded this manner induce brisk proliferation of HDK-1 T (KLH-specific Th1 clone) 5S8 (DNBS-specific Th0 the presence Ag. Our purpose was determine whether CSF-1-dependent mitotic potential might be affected upon Ag-dependent interaction with these clones. Both surface CSF-1R expression responsiveness CSF-1 became undetectable within 24 h after incubation each clone relevant By contrast, alone or Ag had minimal effect, indicating requirement for both Exposure fresh supernatant collected complete XS52/HDK-1/KLH XS52/5S8/DNBS coculture sufficient abrogate responsiveness. Importantly, were restored by mAb against IFN-gamma, diminished rIFN-gamma absence Thus, which detected relatively large amounts above supernatants, serves as major mediator. reduced number binding sites on surface, without affecting mRNA expression. it appears IFN-gamma down-regulates post-transcriptional mechanism. interpret results document novel, bi-directional signaling event DC-T promotes growth but inhibits DC.