Endogenous IL-10 protects mice from death during septic peritonitis

• DOI: 10.4049/jimmunol.155.11.5397
• Publication Date: 2022-12-31T12:18:14Z
• Authors: T van der Poll; A Marchant; W A Buurman; L Berman; C V Keogh; D D Lazarus; L Nguyen; M Goldman; L L Moldawer; S F Lowry
• Keywords: Interleukin-10 -- immunology; Molecular Sequence Data; Peritonitis; Cytokines -- blood; Inbred C57BL; Sepsis -- immunology; Cecum -- surgery; Mice; Sepsis; Sepsis -- etiology; Animals; RNA, Messenger; Cecum; Sepsis -- mortality; DNA Primers; Base Sequence; Peritonitis -- mortality; Peritonitis -- immunology; Sciences bio-médicales et agricoles; Interleukin-10; 3. Good health; Mice, Inbred C57BL; Messenger -- analysis; Peritonitis -- complications; RNA; Cytokines; Female

Abstract IL-10 production during endotoxic shock is part of a protective mechanism that involves IL-10-induced inhibition of TNF synthesis. We sought to determine the role of IL-10 in septic peritonitis induced by cecal ligation and puncture (CLP). CLP led to a rapid induction of IL-10 mRNA in various organs of C57BI/6 mice. In liver, IL-10 mRNA was detectable within 1 h following CLP, while in spleen and lungs, IL-10 mRNA was detected from 2 to 4 h and onward. IL-10 protein became detectable in plasma 2 h after CLP, reaching peak concentrations after 12 h (12.7 +/- 5.7 ng/ml). Pretreatment (-2 h) with anti-IL-10 mAb resulted in higher plasma TNF levels following CLP when compared with mice treated with control mAb. Plasma IL-1 activity and IFN-γ remained undetectable in virtually all mice. Anti-IL-10 enhanced mortality after CLP (p < 0.05 by log-rank test). Addition of anti-TNF mAb did not influence the increased mortality associated with anti-IL-10 treatment. Septic peritonitis is associated with sustained production of IL-10 in various organs, which serves to protect the host against lethality.