Eosinophil recruitment following allergen challenge is associated with the release of the chemokine RANTES into asthmatic airways.

• DOI: 10.4049/jimmunol.157.4.1806
• Publication Date: 2022-12-31T13:25:35Z
• Authors: L M Teran; N Noso; M Carroll; D E Davies; S Holgate; J M Schröder
• Keywords: Adult; Male; 0301 basic medicine; Chemotactic Factors, Eosinophil; 610; Allergens; Asthma; Bronchial Provocation Tests; 3. Good health; Eosinophils; Leukocyte Count; 03 medical and health sciences; Forced Expiratory Volume; Eosinophilia; Humans; Female; Bronchoalveolar Lavage Fluid; Chemokine CCL5

Eosinophil infiltration of the airways in response to allergen exposure is a characteristic bronchial asthma. However, mechanisms by which these cells are recruited poorly understood. We have investigated presence eosinophil chemotactic activity (ECA) bronchoalveolar lavage fluid obtained from allergic asthmatics (n = 6) 4 h after endobronchial challenge. ECA was purified sequential heparin affinity chromatography and reverse-phase HPLC. A single peak detected; SDS-PAGE Western blot analysis showed that contained protein 8 kDa corresponded chemokine RANTES (regulated upon activation, normal T cell expressed secreted). Consistent with this, neutralized an Ab RANTES. Measurement ELISA 10x concentrated increased levels this at site (median, 187 pg/ml; range, 46-263 pg/ml) comparison saline challenge control 32.5 11-94 pg/ml), P < 0.005. Furthermore, there significant correlation between concentrations immunoreactive number eosinophils (r 0.8; p 0.001), but not 0.2; 0.12). These results suggest involved recruitment into asthmatic