Myelin Antigen-Specific CD8+ T Cells Are Encephalitogenic and Produce Severe Disease in C57BL/6 Mice

• DOI: 10.4049/jimmunol.166.12.7579
• Publication Date: 2014-04-20T23:36:18Z
• Authors: Deming Sun; John N Whitaker; Zhigang Huang; Di Liu; Christopher Colec...; Hartmut Wekerle; Cedric S Raine
• Keywords: Adoptive Cell Transfer; Myelin oligodendrocyte glycoprotein; Encephalomyelitis

Encephalitogenic T cells that mediate experimental autoimmune encephalomyelitis (EAE) are commonly assumed to be exclusively CD4+, but formal proof is still lacking. In this study, we report synthetic peptides 35-55 from myelin oligodendrocyte glycoprotein (pMOG(35-55)) consistently activate a high proportion of CD8+ alphabetaTCR+ encephalitogenic in C57BL/6 (B6) mice. The potential MOG-specific was established by adoptive transfer CD8-enriched cells. These induced much more severe and permanent disease than actively immunization with pMOG(35-55). CNS lesions pMOG(35-55) cell-induced EAE were progressive destructive. strongly pathogenic syngeneic B6 RAG-1(-/-) mice, not isogeneic beta2-microglobulin-deficient could repeatedly reisolated for up 287 days recipient or mice which adoptively <1 x 10(6) sensitized It postulated MOG induces relapsing and/or pattern eliciting cell response dominated autoreactive Such appear have an enhanced tissue-damaging effect persist the animal long periods.