Production of Type I IFN Sensitizes Macrophages to Cell Death Induced by Listeria monocytogenes

• DOI: 10.4049/jimmunol.169.11.6522
• Publication Date: 2014-04-20T23:41:24Z
• Authors: Silvia Stockinger; Tilo Materna; Dagmar Stoiber; Lourdes Bayr; Ralf Steinborn; Thomas Kolbe; Hermann Unger; Trinad Chakraborty; David E Levy; Mathias Müller; Thomas Decker
• Keywords: Listeriolysin O; Intracellular parasite; Virulence factor; STAT1

Abstract Type I IFNs (IFN-α/β) modulate innate immune responses. Here we show activation of transcription factor IFN regulatory 3, the synthesis large amounts IFN-β mRNA, and type signal transduction in macrophages infected with Listeria monocytogenes. Expression bacterial virulence protein listeriolysin O was necessary, but not sufficient, for efficient production. Signaling through a pathway involving receptor Stat1 sensitized to L. monocytogenes-induced cell death manner requiring inducible NO synthase (nitric oxide 2) or kinase R, potential effectors action during microbial infections. The data stress importance course infections intracellular bacteria suggest that factors other than contribute macrophage infection.