B Cell Positive Selection by Soluble Self-Antigen

• DOI: 10.4049/jimmunol.169.8.4198
• Publication Date: 2014-04-21T00:03:53Z
• Authors: Sylvie Julien; Pauline Soulas; Jean-Claude Garaud; Thierry Martin; Jean-Louis Pasquali
• Keywords: Immunoglobulin D; Negative selection; Clonal deletion

Abstract It is well established that autoreactive B cells undergo negative selection. This stands in paradox with the high frequency of so-called natural producing low affinity polyreactive autoantibodies recurrent specificities, suggesting these are selected on basis their autoreactivity. We previously described two transgenic mouse lines (with and without IgD) a human autoantibody (nAAb) binds ssDNA Fcγ. In absence IgG, nAAb-transgenic develop normally. By crossing mice animals expressing knockin chimeric IgG Fcγ, we now show constitutive expression promotes increase nAAb-expressing cells. positive selection critically dependent presence IgD, occurs spleen, concerns all mature cell subsets, relative preferential enrichment marginal zone These data support view soluble self-Ags can result clonal