Systemic Administration of IL-15 Augments the Antigen-Specific Primary CD8+ T Cell Response Following Vaccination with Peptide-Pulsed Dendritic Cells

Cytotoxicity, Immunologic Interleukin-15 0301 basic medicine Cell Death Egg Proteins Epitopes, T-Lymphocyte Cell Differentiation Mice, Transgenic Dendritic Cells CD8-Positive T-Lymphocytes Lymphocyte Activation Adoptive Transfer Immunophenotyping 3. Good health Mice, Inbred C57BL Mice 03 medical and health sciences Adjuvants, Immunologic Injections, Intravenous Animals Humans Immunologic Memory Injections, Intraperitoneal
DOI: 10.4049/jimmunol.169.9.4928 Publication Date: 2014-04-21T00:57:06Z
ABSTRACT
The systemic administration of IL-2 can act as a potent adjuvant for T cell-directed vaccine strategies. However, not only is the potentially toxic, but recent evidence suggests that it may also paradoxically limit duration and magnitude cytotoxic cell response. A recently identified cytokine, IL-15, shares many properties with provide preferential means augmenting responses. Although well characterized in vitro, there are few data on ability IL-15 to augment cell-mediated responses vivo. We therefore evaluated function murine model. To establish population easily identifiable Ag-responsive cells, naive CD8(+) (OT-1) cells were first adoptively transferred into mice. Vaccination peptide-pulsed dendritic induced modest expansion OT-1 cells. addition 7 days following vaccination resulted significant increase responding PBL, spleen, lymph nodes. Importantly, maintained Tc1-biased phenotype. did observe either enhanced resistance activation-induced death or generation memory result treatment compared IL-2. These studies show time capable primary response contribute basis future experiments exploring clinical role IL-15.
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