Analysis of Transcription Factor Expression during Discrete Stages of Postnatal Thymocyte Differentiation

• DOI: 10.4049/jimmunol.173.2.1094
• Publication Date: 2014-04-20T23:37:22Z
• Authors: Sahba Tabrizifard; Alexandru Olaru; Jason Plotkin; Mohammad Fallahi-...; Ferenc Livak; Howard T. Petrie
• Keywords: Thymocyte; Transcription

Abstract Postnatal T lymphocyte differentiation in the thymus is a multistage process involving serial waves of lineage specification, proliferative expansion, and survival/cell death decisions. Although these are believed to originate from signals derived various thymic stromal cells, ultimate consequence induce transcriptional changes that definitive each step. To help characterize this process, high density microarrays were used analyze transcription factor gene expression RNA progenitors at stage lymphopoietic differentiation, results validated by number appropriate methods. We find large factors be expressed developing lymphocytes, including many with known roles control proliferation, or cell survival/death decisions other types. Some throughout developmental whereas others change substantially specific transitions. The latter particularly interesting, because stage-specific make it increasingly likely corresponding may involved processes. Overall, data presented here represent resource for discovery confirmation obtained through