Prostaglandin E2 Is Generally Required for Human Dendritic Cell Migration and Exerts Its Effect via EP2 and EP4 Receptors

• DOI: 10.4049/jimmunol.176.2.966
• Publication Date: 2014-04-20T23:43:16Z
• Authors: Daniel F. Legler; Petra Krause; Elke Scandella; Eva Singer; Marcus Groettrup
• Keywords: Prostaglandin E2 receptor

Abstract The control of dendritic cell (DC) migration is pivotal for the initiation cellular immune responses. In this study, we demonstrate that human monocyte-derived (Mo)DCs as well ex vivo peripheral blood DCs toward CCL21, CXCL12, and C5a stringently dependent on presence proinflammatory mediator PGE2, although expressed CXCR4 C5aR their surface DC maturation was accompanied by CCR7 up-regulation independently PGE2. necessity exogenous PGE2 not due to suppression synthesis IL-4, which used MoDC differentiation, because maturation-induced endogenous production cannot promote migration. Surprisingly, absolutely required at early time points enable chemotaxis, whereas addition during terminal events ineffective. contrast mouse DCs, exclusively rely EP4 receptor triggering migration, MoDCs require a signal mediated EP2 or either alone in combination. Our results provide clear evidence general mandatory factor development migratory phenotype myeloid DCs.