Thiocyanate Transport in Resting and IL-4-Stimulated Human Bronchial Epithelial Cells: Role of Pendrin and Anion Channels

• DOI: 10.4049/jimmunol.178.8.5144
• Publication Date: 2014-04-18T22:55:39Z
• Authors: Nicoletta Pedemonte; Emanuela Caci; Elvira Sondo; Antonella Caputo; Kerry Rhoden; Ulrich Pfeffer; Michele Di Candia; Roberto Bandettini; Roberto Ravazzolo; Olga Zegarra-Moran; Luis J V Galietta
• Keywords: Thiocyanate; Pendrin; Lactoperoxidase

SCN(-) (thiocyanate) is an important physiological anion involved in innate defense of mucosal surfaces. oxidized by H(2)O(2), a reaction catalyzed lactoperoxidase, to produce OSCN(-) (hypothiocyanite), molecule with antimicrobial activity. Given the importance availability airway surface fluid, we studied transepithelial transport human bronchial epithelium. We found evidence for at least three mechanisms basolateral apical flux. cAMP and Ca(2+) regulatory pathways controlled through cystic fibrosis transmembrane conductance regulator Ca(2+)-activated Cl(-) channels, respectively, latter mechanism being significantly increased treatment IL-4. Stimulation IL-4 also induced strong up-regulation electroneutral SCN(-)/Cl(-) exchange. Global gene expression analysis microarrays functional studies indicated pendrin (SLC26A4) as protein responsible this transport. Measurements H(2)O(2) production cells that extent modulate conversion oxidant lactoperoxidase system. Our indicate epithelium expresses various under resting stimulated conditions. Defects airways may be susceptibility infections and/or decreased ability scavenge oxidants.