Characterization of the mechanism by which L-asparaginase II of Salmonella enterica serovar Typhimurium induces T cell inhibition (99.12)

• DOI: 10.4049/jimmunol.186.supp.99.12
• Publication Date: 2023-01-01T05:20:06Z
• Authors: AnnMarie Torres; Amy Kullas; Adrianus van der ...
• Keywords: 0303 health sciences; 03 medical and health sciences

Abstract T cells play a key role in controlling and clearing infection with the bacterial pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium). However, T cell-mediated immunity against S. Typhimurium takes months to develop and has been described as slow and inefficient. Previously, we have shown that S. Typhimurium have a direct inhibitory effect on T cells, down-modulating expression of the beta chain of the T cell receptor (TCR-beta) and inhibiting T cell proliferation. Furthermore, we have shown that a soluble, proteinaceous factor produced or induced by S. Typhimurium is responsible for this inhibition. Most recently, we have found that STM3106 is required for S. Typhimurium to inhibit T cells and that L-asparaginase II, which is encoded by STM3106, is present in the supernatants of T cells cultured in the presence of S. Typhimurium. In addition, we have found that L-asparaginase II produced by S. Typhimurium is both necessary and sufficient for the inhibition of T cells. With further characterization, this research should provide new insight into the mechanism by which S. Typhimurium inhibit T cells, avoiding immune clearance.