AhR ligands differentially affect thymus and peripheral lymphoid organs by targeting T cells and their functions leading to pro- or anti-inflammatory responses

• DOI: 10.4049/jimmunol.202.supp.183.4
• Publication Date: 2023-01-01T12:41:47Z
• Authors: Narendra Prasad S...; Kathryn Miranda; Dominique Jackson; Steilan Sumpter; Udai P Singh; Mitzi Nagarkatti; Prakash Nagarkatti
• Keywords: Regulatory T cell

Abstract TCDD, FICZ, and Resveratrol (RES) are potent ligands of aryl hydrocarbon receptor (AhR). TCDD is well known to cause thymic atrophy but the effects FICZ RES have not been studied date. In current study, we investigated effect AhR in vivo. We chose these for their different origin-TCDD, an environmental contaminant, endogenously generated, RES, found plant products. To this end, C57BL/6 mice were treated with vehicle (corn oil) or (10 μg/kg bw) (100 mg/kg by oral gavage. Three days post treatment, thymi spleens harvested stained fluorochrome-labelled Abs against various T cell surface markers analyzed flow cytometry. observed significant on decreasing weight cellularity, when compared vehicle. other hand, had no thymus. however, significantly caused increase as cellularity. Upon analysis populations thymus, downregulation almost all (CD3, CD4, CD8, CD25, CD44, αβTCR) whereas, moderate subpopulations. spleen, three mixed effects. generation Tregs, suppressed Tregs increased Th17 cells. Together, our studies suggest that although ligands, they both thymus spleen. role inducing immune suppression generating more a inflammation