Pathogen-elicited memory γδ T cells express a broadly reactive canonical TCR or a pathogen-specific non-canonical TCR

• DOI: 10.4049/jimmunol.204.supp.231.11
• Publication Date: 2023-01-01T13:28:50Z
• Authors: Camille F Khairallah; Julie Bettke; Jessica Nancy Imp...; Yue Zhang; Zhijuan Qiu; Oleksandr Gorbats...; Kyungjin Cho; Kwang Soon Kim; Lynn Puddington; Charles D Surh; James B Bliska; Adrianus W M Van ...; Brian S Sheridan
• Keywords: Priming (agriculture); Memory T cell; Mesenteric lymph nodes

Abstract γδ T cells are critical sentinels protecting barrier surfaces against pathogens. However, our understanding of cell specificity remains limited. We previously identified a resident memory Vγ4Vδ1 population elicited by foodborne Listeria monocytogenes (Lm) infection. Analysis the CDR3δ sequences revealed that large subset non-canonical clones expanded during primary Lm infection, contracted homeostasis and recalled after secondary infection suggesting antigenic-specificity may be involved in this response. most response was bearing canonical Vδ1 TCR many Lm-elicited not Lm-specific. Indeed, stimulation mesenteric lymph node from Lm-immune mice with diverse pathogens induced functional cells. Similarly, induction Salmonella colitis (STm-colitis) resulted TCR-dependent recall-like response, demonstrating majority these respond to heterologous Interestingly, responded did expand STm-colitis some level is imparted priming pathogen. Importantly, other enteropathogens triggered Adaptive were also generated germ-free bacterial infections SPF mice, indicating can broad range disseminating provide target for universal mucosal vaccines gut.