Analysis of Gluten Proteins Composition During Grain Filling in Two Durum Wheat Cultivars Submitted to Two Water Regimes

• DOI: 10.4081/ija.2014.558
• Publication Date: 2014-02-04T10:58:46Z
• Authors: Marcella Michela ...; Michele Andrea De...; Marianna Pompa; Luigia Giuzio; Zina Flagella
• Keywords: Glutenin; Plant protein

Durum wheat (Triticum turgidum L. subsp. durum) is one of the major crops in Mediterranean basin, where water stress often occurs during grain filling which represents a critical stage for synthesis and accumulation storage proteins (gliadins glutenins). The aim study to evaluate, by two-dimensional gel electrophoresis (2DE SDS-PAGE), composition two durum cultivars (Ciccio Svevo) cultivated growth chamber under different regimes (control deficit). At milk physiological maturity, gluten have been extracted separated 2DE SDS-PAGE. analysis gels was performed software ImageMaster 2D Platinum (Amersham). results showed differences protein expression within regions between cultivars; deficit rate faster all regions, either at stage. Protein high molecular weight (H) region resulted Ciccio than Svevo mainly control treatment. In low 48 35 kDa (L 48-35), cultivar higher Svevo. Furthermore marked increase H volume decrease L 48-35 observed only Svevo; instead no change showing this greater stability on changing regime. Further studies use mass spectrometry are necessary identify specific peptides relative drought as well investigate relationships with technological quality.