Changes in motility, morphology, plasma membrane and acrosome integrity during stages of cryopreservation of buck sperm

• DOI: 10.4102/jsava.v85i1.972
• Publication Date: 2014-02-28T07:50:52Z
• Authors: Mushtaq Ahmad; Rashad Nasrullah; Hasan Riaz; Abdul Sattar; Nasim Ahmad
• Keywords: Dilution; Membrane integrity

Changes in sperm structure and function occur during the processing of semen. The present study was designed to investigate effect on buck different stages semen preparation including dilution, cooling, equilibration freeze-thawing. Semen ejaculates from three mature bucks (replicates = 5) were diluted with tris-citric acid egg yolk glycerol extender at 37 ºC, cooled 4 ºC over 90 min, equilibrated for 2 h, transferred 0.5 mL straws, placed nitrogen vapour, frozen thawed then analysed. Sperm samples assessed percentage motility, acrosomal plasma membrane integrity, live sperm, morphology after thawing. Mean motility dilution (86.0 ± 1.4%) reduced significantly (p < 0.05) due cooling (77.6 1.3% 74.6 1.4% respectively); furthermore, it decreased freezing thawing (42.3 2.5%). higher (89.3 1.4%)compared (84.8 1.8%) (80.2 2.5%) further (56.0 3.4%). dropped 96.4 0.3% 88.8 (81 1.9%). normal (82.2 1.1%) or (73.8 1.8) (50.1 2.9%). acrosomes did not differ (85.8 1.7%, 83.2 1.6%, 81.7 but (45.2 2.8%). In conclusion, showed maximum damage morphology, acrosome integrity following cooling.