Prophylactic Antitumor Effect of Mixed Heat Shock Proteins/Peptides in Mouse Sarcoma

• DOI: 10.4103/0366-6999.162516
• Publication Date: 2015-08-10T11:49:15Z
• Authors: Yu Wang; Shu-Yun Liu; Mei Yuan; Yu Tang; Quan-Yi Guo; Xue-Mei Cui; Xiang Sui; Jiang Peng
• Keywords: ELISPOT; HSP60; Cancer vaccine

To develop a vaccine-based immunotherapy for sarcoma, we evaluated mixture of heat shock proteins (mHSPs) as vaccine sarcoma treatment in mouse model. Heat protein/peptides (HSP/Ps) are autoimmune factors that can induce both adaptive and innate immune responses; HSP/Ps isolated from tumors antitumor activity when used vaccines.In this study, the effects mHSP/Ps on prophylactic immunity. We extracted mHSP/Ps, including HSP60, HSP70, GP96, HSP110, cell lines S180 MCA207 using chromatography. The immunity induced by was assessed flow cytometry, ELISPOT, lactate dehydrogenase release, enzyme-linked immunosorbent assay.Of sarcoma-bearing mice immunized with cells, 41.2% showed tumor regression long-term survival, growth inhibition rate 82.3% at 30 days. Of 50% survival 79.3%. All control died within 40 proportions natural killer CD8+, interferon-γ-secreting cells tumor-specific cytotoxic T-lymphocyte were increased group.Vaccination polyvalent mHSP/P cancer an immunological response marked to autologous tumors. This exerted greater than did or lysates alone.