First Positive Detection of H9 Subtype of Avian Influenza Virus Nucleic Acid in Aerosol Samples from Live Poultry Markets in Guangxi, South of China

• DOI: 10.4103/0366-6999.182824
• Publication Date: 2016-05-24T07:20:08Z
• Authors: Ning Kang; Min Chen; Fu-Yin Bi; Min-Mei Chen; Yi Tan
• Keywords: Nucleic acid detection

INTRODUCTION Avian influenza has become a serious public health problem. Risk factors for human cases are direct or closed contact with ill, died poultry and live markets (LPMs) exposure. There is high risk of infection frequency visiting LPMs.[1] Robust data animal models have provided support the potential virus transmission by aerosol. The experimental showed that aerosol accounts approximately half A spread events.[2] Thus, identifying circulating viruses in environmental samples, especially LPMs, necessary evaluating threat taking preventive measure. present study aimed to analyze distribution avian (AIVs) other styles samples. METHODS hundreds LPMs Nanning, which opened daily. We collected samples Langxi LPM, were one biggest famous 12 stalls Qingxiu district. Aerosol random stall (including chickens, geese, ducks) among every week. They SKC SETI BioSampler (SKC, US PATENT #5,902,385)[3] at 1.5 m 9 ml viral sampling medium (Yocon MT0301-1, China) 1h L/min. At same time, floor sewage, swab cage chopping block, feather stored 4°C. Viral RNA was extracted from specimens using mini kit (Qiagen 74104, Germany). Real-time polymerase chain reaction performed nuclei acid detected (BioPerfectus Technologies, JC10103, China). Samples tested positive then analyzed H5/H7/H9 subtype JC10301, Serum occupational population antibody levels H5N1 H7N9 hemagglutination inhibition tests, respectively. Data entered into customized database (Microsoft Excel 2007, Microsoft, USA) transferred SPSS (version 16.0, Inc., Chicago, IL, analysis. RESULTS Overall, 699 1526 (45.81%) [Table 1]. Influenza all kinds found rate significant difference between distinct style (χ2 = 51.373, P 0.000 2013; χ2= 32.219, 0.000, 2014).Table 1: Results real-time RT-PCR testing China, April 2013 December 2014The research 30 75 (40.00%) acid, six (20.00%) H9 subtypes AIV 24 (80.00%) Except both H5 higher than 20.47, < 0.01), contrast, 2014 63.12, 0.01). No sample H7 AIV. As shown Figure 1a, H5, had great diversity each month.Figure (a) Percent monthly during 2014. (b) Positive 2014.There (for 2013, 84.272, 0.000; 2014, 72.751, 0.000) autumn winter [Figure 1b], consistent previous results. It 840 470 serums negative antibody, DISCUSSION results suggest nucleic exist Guangxi. might be an important mode infected after LPMs; thus, it needed monitor LPMs. Previous studies closely linked H9N2, H5N1, 2003,[4]2006, 2013,[5] promote human. still confirm transmitted mechanism through exposure Chickens successfully H5N1and H9N2 caused titers virus.[67] been isolated animals LPMs[891011] air chicken houses China.[12] Our first reported existing LPM Guangxi, providing LPM. not while four further investigate whether there differences viability play role dissemination AIVs, active surveillance should carried out as early warning system outbreaks. widespread its reassortment credible future.[9] specimens. possible reasons low survival concentration BioSampler. serological 2 years suggested no recessive AIVs population, although prevailed environment PLM Guangxi recent years. implies infect limited. Financial sponsorship This work supported grant self-raised funds scientific subject Health Family Planning Commission (No. Z2015457). Conflicts interest conflicts interest. Acknowledgments would like thank Dr. Hong-Man Zhang language editing paper revision on behalf Zhuang Autonomous Region Disease Prevention Control.