Bone alkaline phosphatase and osteocalcin expression of rat's Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study)

• DOI: 10.4103/ejd.ejd_261_18
• Publication Date: 2018-10-03T10:23:25Z
• Authors: Alexander Patera ...; Ida Bagus Narmada; Diah Savitri Erna...; Aristika Dinaryanti; Eryk Hendrianto; Wibi Riawan; Fedik Abdul Rantam
• Keywords: CD90

ABSTRACT Objective: The aim of this study was to analyze the osteogenic differentiation rat GMSCs cultured in PRF for bone remodeling. Materials and Methods: were isolated from lower gingival tissue four healthy, 250 g, 1-month old, male rats (Rattus norvegicus) cut into small fragments, 2 weeks, subsequently passaged every 4–5 days. passage 3 characterized by CD34, CD45, CD44, CD73, CD90, CD105 using fluorescein isothiocyanate immunocytochemistry (ICC) examination. 3–5 five M24 plates (N = 108; n 6/group) 7, 14, 21 days with three different mediums as follows: Control (−) group: α-Modified Eagle Medium; (+) High-dose glucose Dulbecco's Modified Eagle's Medium (DMEM-HG) + medium; treatment DMEM-HG medium PRF. vitro alkaline phosphatase (BALP) osteocalcin (OSC) marker ICC monoclonal antibody. Statistical Analysis Used: one-way analysis variance performed (P < 0.05) based on Shapiro–Wilk Levene's tests > 0.05). Results: shown present +CD73, +CD90, +CD105 − CD45 expression MSCs markers. group showed highest BALP (16.00 ± 1.732) day while OSC (13.67 2.309) statistically significant difference between groups Conclusion: demonstrated potential ability capable accelerating remodeling enhancing expression.