Production of vitamin B12 via microbial strains isolated from marine and food sources in Egypt

• DOI: 10.4103/epj.epj_267_23
• Publication Date: 2024-04-25T05:00:31Z
• Authors: Rania M. M. Abdel...; Galal M. Khalafalla; Olfat S. Barakat; Marwa N. Ahmed
• Keywords: Yeast extract; Industrial microbiology; Vitamin b

Background Vitamin B 12 is a very important water-soluble vitamin, which was first isolated from the liver as an anti-pernicious anemia factor. The sole source of vitamin animal-based food. It has complicated structure and requires expensive multi-steps to be synthesized chemically. Intriguingly, can produced through microbial fermentation by microorganisms in cheap more effective manner. Objective This study aims isolate characterize that have capability produce . In addition, current work optimize production conditions isolating strains using suitable waste materials obtain high yield. Materials methods Different bacterial yeast isolates were marine food samples pour-plate technique. These screened for specific growth medium lacked test indicator bacterium. content estimated spectrophotometer measurement high-performance liquid chromatography (HPLC). showed productivity identified MALDI-TOF implemented optimization reveal most proper optimum production. Response surface methodology (RSM) employed enhance flask scale. Agro-industrial wastes such molasses used determined RSM. Results conclusion Eighty-seven actinomycetes, bacterial, Out these isolates, 15 productivity. We found bacilli productive among tested cocci actinomycetes isolates. highly Bacillus analysis. Candida pelliculosa , Geotrichum candidum subtilis sp One strain (coded BYI), three MZYC, MZYD, MZYG) selected studying effect sugar type inoculum size on yield Strain MZYD statistical modelling RSM seven factors included temperature, time, salt concentration, pH, size, aeration. Five i.e ., shown significantly improve A maximum 64.21 μg/100 ml obtained optimized conditions. raw material growth.