Proteomics-based approach for differentiation of age-related macular degeneration sub-types

• DOI: 10.4103/ijo.ijo_470_20
• Publication Date: 2021-02-17T10:35:03Z
• Authors: Sivapriya Sivagur...; Lakshmi Dhevi N S...; Aafaque Ahmad Khan; Sowmya Parameswaran; Harsha Bhattacharjee; Krishna Gogoi; Harsha Gowda; T. S. Keshava Prasad; Akhilesh Pandey; S Ashok Kumar; Pukhraj Rishi; Ekta Rishi; Dhanashree Ratra; Muna Bhende; Narayanan Janakir...; Jyotirmay Biswas; Subramanian Krish...
• Keywords: Complement component 3

Purpose: Age-related macular degeneration (AMD) is one of the leading causes irreversible central vision loss in elderly population. The current study aims to find non-invasive prognostic biomarkers urine specimens AMD patients. Methods: Peripheral blood and samples were collected from 23 controls 61 Genomic DNA was extracted buffy coat peripheral blood. Allele specific PCR used assay SNPs complement factor H (CFH), component 3 (C3). Comparative proteomic analysis early AMD, choroidal neovascular membrane (CNVM), geographic atrophy (GA), healthy performed using isobaric labelling followed by mass spectrometry. Validation enzyme-linked immunosorbent (ELISA). Results: identified 751 proteins, which 383 proteins found be differentially expressed various groups Gene ontology classification revealed majority them involved catalytic functions binding activities. Pathway showed cell adhesion molecule pathways (CAMs), Complement coagulation cascades, significantly deregulated AMD. Upon validation ELISA, SERPINA-1 (Alpha1 antitrypsin), TIMP-1 (Tissue inhibitor matrix metaloprotease-1), APOA-1 (Apolipoprotein A-1) over-expressed ( n = 61) patients compared 23). A logistic model combination with CFH C3 polymorphisms predicted risk developing 82% accuracy. Conclusion: This gives us a preliminary data on predictive for can further validated large cohort translated diagnostic use.