Circulating free DNA as a marker of response to chemoradiation in locally advanced head and neck squamous cell carcinoma

• DOI: 10.4103/ijpm.ijpm_28_20
• Publication Date: 2020-10-27T16:47:34Z
• Authors: Nuzhat Husain; Tripti Verma; Swati Kumari; Sridhar Mishra; Madhup Rastogi; Vandana Tiwari; GauravR Agarwal; Nidhi Anand
• Keywords: Liquid biopsy; Cell-free fetal DNA; Chemoradiotherapy

Liquid biopsy has moved from bench to bedside as a non-invasive biomarker for early diagnosis and monitoring treatment response.This study investigated the role of circulating free DNA (cfDNA) diagnostic marker in locally advanced head neck squamous cell carcinoma (HNSCC) response chemoradiation therapy.Serum was collected naïve, histopathologically diagnosed tumors 24 HNSCC cases 16 normal controls. CfDNA levels were quantified using β globin gene amplification.The cfDNA level significantly elevated (992.67 ± 657.43 ng/mL) compared healthy controls (60.65 30.42 ng/mL, P = <0.001). The did not correlate with TNM stage, lymph node involvement grade. In responders, percentage decrease 9.57% 29.66%, whereas nonresponders increase 13.28% 24.52% at end three months follow-up.Our adds evidence that are higher provides some tumor progression. may be promising prospective predict patients undergoing chemo-radiotherapy.