Determining the antibacterial substantivity of Triphala mouthwash and comparing it with 0.2% chlorhexidine gluconate after a single oral rinse: A crossover clinical trial

• DOI: 10.4103/jisp.jisp_265_18
• Publication Date: 2018-10-31T11:34:58Z
• Authors: RitamSanvala Naik...; Chandrakant Dharm...; AbhijitNingappa G...; Satish Kakade
• Keywords: 03 medical and health sciences; 0302 clinical medicine; Original Article; 3. Good health

Triphala has been extensively used in dentistry as a mouthwash because of its antiplaque and antigingivitis properties.The present study is designed to determine the duration of its antibacterial substantivity after a single oral rinse and to compare it with the substantivity of 0.2% chlorhexidine gluconate (CHX).In this clinical crossover trial, unstimulated saliva from 30 individuals was collected 2 h after routine oral hygiene procedures but not rinsing (pre-sample) with randomly selected mouthwash, (10% Triphala mouthwash, 0.2% CHX, and normal saline) and 5 min after rinsing (postsample). A washout period of 1 week was kept between two rinses. The sampling was repeated after every 2 h for 12 h (post 1, post 2, post 3, post 4, post 5, and post 6) and was checked for microbial count.Friedman test, Kruskal-Wallis test, and post hoc analysis were used to assess the effect of different mouthrinses on colony forming units at different times.Ten percent Triphala showed statistically significant results when the antibacterial effect at post, post 1 and post 2 were compared to pre-assessment count (P < 0.05). After which the effect was at par with normal saline (P > 0.05). The results for CHX were statistically significant at all times when compared to pre-assessment count (P < 0.05) and it showed the maximum substantivity of 7 h.After a single rinse with no eating and drinking restrictions over the day, 10% Triphala mouthwash had an antibacterial effect for 3-4 h. It can be used three times daily for its maximum antibacterial effect.