Effects of STZ-Induced Diabetes on Zinc, Boron and Antioxidant Defense Mechanisms in Rats

• DOI: 10.4103/njcp.njcp_566_24
• Publication Date: 2025-03-30T04:48:45Z
• Authors: F Demirkaya Miloglu; G Gundogdu; B Bayrak; Y Kadioglu; B Yuksel
• Keywords: Intraperitoneal injection; Pathogenesis; Homeostasis

Background: Diabetes mellitus (DM), characterized by dysregulation of glucose metabolism, is a significant global health issue. This study aims to investigate the effects DM, induced with streptozotocin (STZ), on serum zinc and boron levels as well antioxidant defense mechanisms in rats. Materials Methods: In this study, rat model was utilized where rats, after an overnight fast, were administered single intraperitoneal dose STZ induce type-1 diabetes. Diabetic status confirmed three days post-STZ administration fasting blood exceeding 300 mg/dL. Six rats assigned STZ-induced diabetic (DM group) control groups (C group). Inductively coupled plasma mass spectrometry (ICP-MS) used analyze samples treated hydrogen peroxide nitric acid. Furthermore, analyzed using ELISA measure total oxidant-antioxidant (TOS-TAS). Results: The ICP-MS method validated validation parameters including linearity (10–500 ng/mL), precision (≤ 3.25% RSD), accuracy ±2.58% RE), recovery (98.2 ± 4.53% for 101.4 5.46% boron). Our results showed significantly decreased both DM group compared C ( P = 0.001), suggesting possible link between trace element pathogenesis. statistically increase TOS 0.006); decrease TAS 0.001) group. Assessment oxidative stress demonstrated imbalance homeostasis further implicating role elements DM-associated complications. Conclusion: These findings contribute valuable insights into complex interplay DM.