Identification of three kinds of citri reticulatae pericarpium based on deoxyribonucleic acid barcoding and high-performance liquid chromatography-diode array detection-electrospray ionization/mass spectrometry/mass spectrometry combined with chemometric analysis

• DOI: 10.4103/pm.pm_581_16
• Publication Date: 2018-02-20T09:11:55Z
• Authors: Xinjun Xu; Xiaoxue Yu; Yafeng Zhang; Dongmei Wang; Lin Jiang
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Original Article; 3. Good health

Citri Reticulatae Pericarpium is the dried mature pericarp of Citrus reticulata Blanco which can be divided into "Chenpi" and "Guangchenpi." "Guangchenpi" genuine Chinese medicinal material in Xinhui, Guangdong province; based on greatest quality least amount, it most expensive among others. Hesperidin used as marker to identify described Pharmacopoeia 2010. However, both contain hesperidin so that impossible differentiate them by measuring hesperidin.Our study aims develop an efficient accurate method separate from other Pericarpium.The genomic deoxyribonucleic acid (DNA) all materials was extracted then internal transcribed spacer 2 amplified, sequenced, aligned, analyzed. The secondary structures were created terms database website established Jörg Schultz et al. High-performance liquid chromatography-diode array detection-electrospray Ionization/mass spectrometry (HPLC-DAD-ESI-MS)/MS coupled with chemometric analysis applied compare differences chemical profiles three kinds Pericarpium.A total 22 samples classified groups. results DNA barcoding accordance principal component hierarchical cluster analysis. Eight compounds deduced HPLC-DAD-ESI-MS/MS.This a reliable effective tool regions structure varied considerablyAll analyzed high-performance chromatography (HPLC) obtain profilesPrincipal analysisdeoxyribonucleic HPLC-diode ionization/mass spectrometry/MS provided strong proof these herbs. Abbreviations used: CTAB: Hexadecyltrimethylammonium bromide, DNA: Deoxyribonucleic acid, ITS2: Internal 2, PCR: Polymerase chain reaction.