Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells

• DOI: 10.4103/sjg.sjg_520_18
• Publication Date: 2019-09-26T07:50:38Z
• Authors: Fan Yang; Lei Luo; Yuqing Liu; Xin Cai; Yao Wang; Juan Xue; Juan Zhang
• Keywords: Occludin

This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat cell (IEC) line.LPS used stimulate IEC-18 cells (1 μg/ml), with or without different concentrations BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) measured detect permeability cells. Interleukin-6 (IL-6) tumor necrosis factor-α (TNF-α) supernatant were detected enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) employed mRNA levels zonulae occludens (ZO)-1 occludin. Western blot immunofluorescence analysis analyzing expression level distribution patterns ZO-1 occludin protein.After treatment BSP, IL-6 TNF-α significantly decreased compared experiment group (P < 0.05 0.01). The IEC groups when In addition, group, up-regulated protein occludin, kept intact injured LPS 0.01).BSP has capacity protect from LPS-induced injury. mechanisms may be associated decreasing inflammatory cytokine TNF-α, elevating which might serve as a new protective agent disruption.