Polymeric Nanoparticles Containing Both Antigen and Vitamin D3 Induce Antigen-Specific Immune Suppression

• DOI: 10.4110/in.2019.19.e19
• Publication Date: 2019-06-25T13:17:17Z
• Authors: Ho-Hyun Jung; Sang-Hyun Kim; Jun-Hyeok Moon; Seong-Un Jeong; Sundong Jang; Chan-Su Park; Chong-Kil Lee
• Keywords: 0301 basic medicine; 03 medical and health sciences; Original Article; 3. Good health

The active form of vitamin D3, 1,25-dihydroxyvitamin D3 (aVD3), is known to exert beneficial effects in the treatment autoimmune diseases because its immunosuppressive effects. However, clinical application aVD3 remains limited potential side effects, particularly hypercalcemia. Encapsulation within biodegradable nanoparticles (NPs) would enhance delivery antigen presenting cells, while preventing systemic aVD3. In present study, polymeric NPs containing ovalbumin (OVA) and (NP[OVA+aVD3]) were prepared via water-in-oil-in-water double emulsion solvent evaporation method, after which their immunomodulatory examined. Bone marrow-derived immature dendritic cells (DCs) treated with NP(OVA+aVD3) did not mature into immunogenic DCs but converted tolerogenic DCs, express low levels co-stimulatory molecules MHC class II molecules, produce lower pro-inflammatory cytokines increasing production IL-10 TGF-β, induce generation Tregs. Intravenous injection markedly suppressed OVA-specific CTLs mice. Furthermore, immune tolerance was induced mice orally administered NP(OVA+aVD3). These results show that encapsulating both can effectively antigen-specific suppression.