Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae

• DOI: 10.4142/jvs.2003.4.3.225
• Publication Date: 2019-02-14T04:55:05Z
• Authors: Sung Jae Shin; Jong Lye Bae; Young Wook Cho; Moon Sik Yang; Dae Hyuk Kim; Yong Suk Jang; Han Sang Yoo
• Keywords: Actinobacillus pleuropneumoniae; Primer (cosmetics); Cloning (programming)

Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. Korean isolate by PCR with primer designed based on the N- and C-terminal of toxin was cloned TA cloning vector sequenced. The nucleotide sequences reported to GenBank accession numbers AF363361. Identity Apx IA E. coli proved SDS-PAGE Western blot. Yeast has been demonstrated be excellent host for expression recombinant proteins uses diagnostics, therapeutics vaccine productions. Therefore, use yeast as delivery system new oral subunit vaccine, subcloned into Saccharomyces cerevisiae, identified protein. First, primers containing BamHI SalI site at each end. Second, DNA digested ligated YEpGPD-TER vector, transformed S. cerevisiae 2805. Third, after identification correctly oriented clone, 120-kDa protein expressed 2805