Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera

• DOI: 10.4142/jvs.2016.17.3.307
• Publication Date: 2016-09-26T08:00:11Z
• Authors: Hyojin Lee; Eun-Ju Kim; Jae-Young Song; Jeong Soo Choi; Ji Youn Lee; In-Soo Cho; Yeun-Kyung Shin
• Keywords: Phlebovirus

Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans.This manifests as decreased blood cell count and multi-organ failure, case-fatality rate of more than 12% China.Because vaccines or antiviral drugs for treatment this are not available, monitoring circulation animals controlling tick-mammal cycle important preventing SFTS.Monoclonal antibodies against recombinant nucleoprotein SFTSV were generated to develop competitive enzyme-linked immunosorbent assay (cELISA) detection infection cattle.The specificity sensitivity cELISA was assessed comparing results those immunofluorescence (IFA).The using 416 field bovine serum samples laboratory-immunized positive sera showed 98.1% consistency IFA.The used study did show cross-reactivity antisera other viral cattle diseases.The presented can be applied detect cattle.