α2,3- and α2,6-linked sialic acids are important for cell binding and replication of Newcastle disease virus in chicken primary neuronal cells

• DOI: 10.4149/av_2018_217
• Publication Date: 2018-08-30T10:08:58Z
• Authors: Y. GUO; J. ZHAO; X. CHANG; W. YAO; H. WANG; W. WANG; X. WANG; S. ZHANG; Z. YANG; S. XIAO
• Keywords: Newcastle Disease; Neurotropic virus; Tissue tropism; Mononegavirales

Velogenic Newcastle disease virus (NDV) causes encephalitis and severe neurological disorders in avian species. Sialic acid (SA) has been considered as a receptor for NDV infection determining tissue tropism. The neurotropic mechanism of birds is completely unknown. Here we have investigated the role viral SA neurotropism chickens. We determined that α2,3- α2,6-linked receptors were implicated binding to primary neuronal cells using immunohistofluorescence virus-cell assay. Both found co-localize with velogenic strain F48E9 neuropathological lesions chicken brains after through intraocular-nasal routes. replication was more efficient than lentogenic LaSota. virus-neuronal cell capabilities both strains no difference. Furthermore, cell-binding capability significantly decreased by pretreatment sialidases cells. These results demonstrated important initiation nervous system. This study should provide preliminary evidence better understanding