Time-course of transcriptome response to respiratory syncytial virus infection in lung epithelium cells

• DOI: 10.4149/av_2018_225
• Publication Date: 2018-08-30T10:08:58Z
• Authors: S. AMPUERO; R. ANDAUR; M. MILANO; M. MORENO; L. LIZAMA; C. LARRAÑAGA; U. URZÚA
• Keywords: 0301 basic medicine; 03 medical and health sciences; Time Factors; Gene Expression Profiling; Respiratory Syncytial Virus, Human; Humans; Epithelial Cells; Respiratory Syncytial Virus Infections; Transcriptome; Lung; 3. Good health

Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection in infants. Winter outbreaks Chile result 5% infected children hospitalized, with 0.01% mortality. Increased evidence indicates that viral and host factors modulate severity infection. Using DNA microarrays, we characterized genome-wide transcriptional response lung mucoepidermoid cells (NCI-H292) at 0, 24, 48, 72 96 hours post-infection (hpi) a single dose RSV/A. During whole studied period, bi-phasic gene expression profile was observed by total 330 differentially expressed genes. About 60% them were up-regulated between 24-72 hpi then turned-off hpi. This transient, early pattern significantly enriched biological processes like interferon signaling, antigen processing presentation, double-stranded RNA binding chemokine activity. We detected 27 common genes hpi, from which IFIT1, IFI44, MX1, CXCL11 OAS1 had highest expression. The second comprised over 120 genes, remained silenced until but steeply Biological this late-response included cell cycle division microtubule cytoskeleton organization. Conversely, belonging to pathway showed decreased conclude RSV induces an innate immune activation Thereafter, inhibited, leading recovery. presented cellular model allows study specific pathways involved elimination prolonged time intervals their subsequent analysis severe disease infants and/or older adults.