Secondary dengue infection in immunocompetent murine model leads to heart tissue damage

• DOI: 10.4149/av_2019_309
• Publication Date: 2019-09-10T09:00:34Z
• Authors: F. C. JÁCOME; A. L. TEIXEIRA; D. D. COUTINHO; A. DA COSTA; G. C. CALDAS; M. A. NUNES; O. M. BARTH; D. F. BARRETO-VIEIRA
• Keywords: Dengue; 0301 basic medicine; Disease Models, Animal; Mice; Mice, Inbred BALB C; 03 medical and health sciences; Coinfection; Myocardium; Animals; Heart; Dengue Virus; 3. Good health

Dengue, considered the most important arthropod-borne viral disease affecting humans, is transmitted by bite of mosquitoes genus Aedes and caused one four distinct serotypes dengue virus (DENV-1, -2, -3 -4). Infection with provides lifelong homotypic immunity. However, immunity against heterologous transient. As a consequence, secondary infection may lead to severer manifestations due cross-reactivity antibodies T-cells. Over 500,000 people are hospitalized every year around 2,5 million, living in endemic areas, at risk infection. Given background, development vaccines anti-DENV drugs utmost importance, as characterization an animal model for testing them. The purpose this study was investigate ultrastructural alterations DENV BALB/c mice heart. To achieve our goal, six were infected DENV-1 and, 4 months later, reinfected DENV-2. Uninfected used negative controls. Heart samples collected processed histopathological analysis. Our results showed edema, endothelium activation characterized presence transport vesicles, free platelets interstitium, mitochondria presenting rarefied matrix degenerated cristae, disorganization muscle fibers. These point not only susceptibility infection, but also fact that, although it often reported occurrence, can heart damage. Keywords: dengue; experimental model; reinfection; mice.