Choline and citicoline ameliorate oxidative stress in acute kidney injury in rats

• DOI: 10.4149/bll_2023_007
• Publication Date: 2022-12-15T10:29:53Z
• Authors: Elif BARIS; Oguzhan SIMSEK; Mualla Aylin ARICI; Metiner TOSUN
• Keywords: Choline; NOX4; Citicoline; P22phox; Microdialysis

The purpose of this study is to investigate the effects cholinergic anti-inflammatory pathway (CAP)-activating drugs, choline and citicoline (Cytidinediphosphate-choline, CDP-choline), on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) parameters contribution NADPH Oxidase4 (NOX4) p22phox.Endotoxemia induces a systemic inflammatory response characterized by production pro-inflammatory mediators reactive oxygen species (ROS), which eventually develops (AKI). p22phox contributes development endotoxemia-induced AKI. Inflammatory can be controlled CAP.Expressions levels KIM-1, TNF-α, NOX4, NFκB in tissues rats were analyzed via RT-PCR experimental groups; 1. Control, 2. LPS (10 mg/kg) + saline, 3. CDP-choline (375 4. (90 mg/kg). Choline ROS also measured spectrofluorometric assay.LPS-induced elevations decreased or administration (p < 0.001). LPS-elevated TNFα, p22 phox, expressions significantly treatments 0.001).Decreased treatment groups suggests that may have therapeutic potential endotoxemia-associated AKI downregulating NOX4 (Tab. 1, Fig. 5, Ref. 45). Text PDF www.elis.sk Keywords: endotoxemia, choline, cytidine diphosphate injury, species.