Hypericin fluorescence kinetics in the presence of low density lipoproteins: study on quail CAM assay for topical delivery
Anthracenes
0301 basic medicine
Metabolic Clearance Rate
Administration, Topical
Neoplasms, Experimental
Quail
Chorioallantoic Membrane
Fluorescence
3. Good health
Lipoproteins, LDL
Kinetics
03 medical and health sciences
Cell Line, Tumor
Animals
Humans
Biological Assay
Perylene
Fluorescent Dyes
DOI:
10.4149/gpb_2016014
Publication Date:
2016-10-07T09:38:58Z
AUTHORS (8)
ABSTRACT
There has been increasing interest in fluorescence-based imaging techniques in clinical practice, with the aim to detect and visualize the tumour configuration and the border with healthy tissue. Strong photodynamic activity of hypericin (Hyp) can be improved by various molecular transport systems (e.g. LDL). Our aim was to examine pharmacokinetics of Hyp in the presence of LDL particles on ex ovo chorioallantoic membrane (CAM) of Japanese quail with implanted TE1 tumour spheroids (human squamocellular carcinoma). Spheroids were implanted on CAM surface on embryonal day 7 and after 24 hours formulations of free Hyp and Hyp:LDL 100:1 and 200:1 were topically applied. All experimental formulations in the fluorescent image very well visualized the tumour spheroid position, with gradual increase of fluorescence intensity in 6-h observation period. LDL transportation system exhibited clear superiority in fluorescence pharmacokinetics than free Hyp formulation by increasing tumour-normal difference. Our experimental results confirm that Hyp and Hyp:LDL complex is potent fluorophore for photodynamic diagnosis of squamocellular carcinoma.
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