Clonal analysis of gastric carcinoma and precancerous lesions and its relation to Ki-67 protein expression

• DOI: 10.4149/neo_2009_01_48
• Publication Date: 2009-06-08T19:43:28Z
• Authors: L. Wang; L. Zheng; S. Wang; T. Zhu; H. Zhu
• Keywords: Intestinal metaplasia; Laser capture microdissection; Atrophic gastritis; Microdissection; Ki-67

The pathogenesis of intestinal carcinoma is characterized as progressing through multiple steps, which begin with atrophic gastritis followed by metaplasia, dysplasia and carcinoma. However, the clonal status gastric precancerous lesions its association proliferative kinetics have not been fully understood. In this study, normal epithelial cells were isolated from formalin-fixed paraffin embedded tissues using a laser capture microdissection (LCM) system, clonality was analyzed human androgen receptor gene (HUMARA) polymerase chain reaction (PCR), PCR products examined Applied Biosystems 3730 DNA Analyzer. relationship between Ki-67 protein expression also investigated. detected two-step immunohistochemical staining. 5/32 metaplasia lesions, 10/45 low grade intraepithelial neoplasia, 25/36 high neoplasia 20/20 monoclonal origin. Similar to inactivation, rate increased along multi-step carcinogenesis. Clonal associated certain extent, may be useful in assessing susceptibility Key words: carcinoma; lesion; analysis; Ki-67.