Overexpression of AEG-1 in renal cell carcinoma and its correlation with tumor nuclear grade and progression

• DOI: 10.4149/neo_2010_06_522
• Publication Date: 2010-10-22T19:42:32Z
• Authors: W. CHEN; Z. KE; H. SHI; S. YANG; L. WANG
• Keywords: Cell Nucleus; Membrane Proteins; RNA-Binding Proteins; Immunohistochemistry; Kidney Neoplasms; 03 medical and health sciences; 0302 clinical medicine; Cell Line, Tumor; Disease Progression; Humans; Carcinoma, Renal Cell; Cell Adhesion Molecules; Neoplasm Staging

The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features survival patients. Real-time reverse transcription-PCR Western blot showed markedly higher AEG-1 8 cases RCC tissue compared paired normal from same patient. level also increased four lines contrast tubular epithelial human kidney cells 2 (HK-2) both mRNA protein levels. Furthermore, immunohistochemistry analysis highly expressed 96 102 (94.1%) paraffin-embedded archival tissue. Statistical significant tumor grade (P <0.001), clinical staging = 0.003), T classification 0.003) as well metastasis (P=0.032). means for time low group 76.98m<br />while high AEG-1expression 60.94m. Our results suggest that is overexpressed plays an important role differentiation progression. <br />High closely associated poor prognosis.Astrocyte gene-1, AEG-1, carcinoma, prognosis.