Receptor-interacting protein kinase 3 is a predictor of survival and plays a tumor suppressive role in colorectal cancer

• DOI: 10.4149/neo_2015_071
• Publication Date: 2015-05-20T05:55:51Z
• Authors: X. FENG; Q. SONG; A. YU; H. TANG; Z. PENG; X. WANG
• Keywords: Propidium iodide; Tissue microarray

Receptor-interacting protein kinase 3 (RIP3) is a member of the RIP Ser/Thr family, plays an important role in regulating cell survival, apoptosis and necrosis. However, RIP3 carcinogenesis colorectal cancer still poorly understood.We used quantitative PCR Western blot analysis to examine expression primary paired normal mucosa. clinicopathological significance was assessed by immunohistochemical staining 112 cases with noncancerous tissues. The biological function overexpression measured CCK8 assay plate colony formation assay. Dual fluorescent Annexin V propidium iodide (PI) discriminate apoptotic or necrotic death.RIP3 significantly lower associated T stage, M stage AJCC stage. Cox proportional hazard models showed that independent prognostic factor for overall survival disease-free patients cancer. Overexpression suppressed proliferation cells vitro.Our results suggest may as novel indicator after surgery play suppressive carcinogenesis.