miR-3662 suppresses cell growth, invasion and glucose metabolism by targeting HK2 in hepatocellular carcinoma cells

• DOI: 10.4149/neo_2020_190730n689
• Publication Date: 2020-07-29T18:13:30Z
• Authors: J. Ye; X. Xiao; Y. Han; D. Fan; Y. Zhu; L. Yang
• Keywords: Ectopic expression

Hepatocellular carcinoma (HCC) is one of the most common malignancies with a rising incidence around world. MicroRNAs (miRNAs) have been reported to play essential roles in progression HCC. However, precise mechanism miR-3662 HCC process remains poorly understood. This study was aimed determine regulatory network and hexokinase 2 (HK2) Quantitative real-time polymerase chain reaction (qRT-PCR) performed detect expression. Cell proliferation invasion were measured by Counting Kit-8 (CCK-8) assay Transwell assay, respectively. Glucose consumption lactate production assays used glucose metabolism activity cells. The potential binding sites between HK2 predicted TargetScan online software relationship verified luciferase report assay. protein expression western blot analysis. A xenograft tumor model established confirm role vivo. downregulated tissues cells, it reduced hypoxia-induced cells time-dependent manner. Overexpression or knockdown inhibited cell proliferation, invasion, which could be reversed upregulating HK2. Besides, direct target hypoxia upregulated In addition, upregulation abolish overexpression-induced inhibitory effects on growth