Evaluation of Sample Extraction Methods for Minimizing Hematocrit Effect on Whole Blood Analysis with Volumetric Absorptive Microsampling

• DOI: 10.4155/bio-2015-0028
• Publication Date: 2017-01-19T16:34:02Z
• Authors: Zhengqi Ye; Hong Gao
• Keywords: Sample (material)

Aim: Volumetric absorptive microsampler (VAMS) was designed to sample a fixed volume of blood regardless the hematocrit (HCT) levels. Model compounds with wide range hydrophobicity were evaluated for their extraction recoveries from VAMS dried samples. Results: For highly hydrophobic compounds, methanol or methanol/acetonitrile higher compared using aqueous mixture acetonitrile. Extraction (1:1) yielded more consistent recovery across HCT 20–70% than alone, good linearity, accuracy and precision achieved 1 2000 ng/ml. Conclusion: An organic solvent preparation approach developed optimize minimize effect on analysis