Quantification of Microdosed Oral Yohimbine and its Major Metabolite in Human Plasma in the Picogram Range

• DOI: 10.4155/bio-2019-0129
• Publication Date: 2019-08-14T12:39:30Z
• Authors: Manuela Vay; Max Sauter; Gerd Mikus; Jürgen Burhenne
• Keywords: 610; Administration, Oral; Analytic Sample Preparation Methods; Reproducibility of Results; Yohimbine; 3. Good health; 03 medical and health sciences; 610 Medical sciences Medicine; 0302 clinical medicine; Limit of Detection; Calibration; Humans; Blood Chemical Analysis

Aim: Pharmacokinetics after oral microdosing of the anticipated CYP2D6 substrate yohimbine and its metabolite 11-OH-yohimbine is potentially useful for drug–drug interaction trials profiling enzyme activity. Materials & methods: We developed an ultrasensitive ultra performance liquid chromatography coupled to tandem mass spectrometry assay quantification main in plasma with a linear calibration range 5–2500 pg/ml validated it according US FDA's EMA's guidelines. Sample preparation was performed using fast liquid–liquid extraction. The applied determination concentrations administration 50 μg two subjects. Conclusion: Ultrasensitive enables their which would be applicable use phenotyping.